School of Food Systems, North Dakota State University, Fargo, ND 58108-5060, USA.
Food Chem. 2012 Dec 15;135(4):2203-6. doi: 10.1016/j.foodchem.2012.06.124. Epub 2012 Jul 14.
Detection of adulteration of split red lentil (Lens culinaris L.) seeds with low level addition of split common vetch (Vicia sativa L.) is hampered by a lack of reliable detection methods. An analytical method was developed using high performance liquid chromatography with diode array detection (HPLC-DAD) based on two unique chemical markers found in common vetch: ß-cyanoalanine (BCA) and γ-glutamyl-ß-cyanoalanine (GCA). These two markers were present in samples of common vetch seed grown in Canada and Serbia. Authentic lentil samples grown in Canada, Australia, USA, Turkey, Syria, and Morocco had no detectable levels of these chemical markers. Commercial lentil samples for export from lentil processing plants in Saskatchewan, Canada, also had no detectable levels of GCA and BCA. The presence of vetch in intentionally adulterated lentil samples could be determined via chemical markers with a detection limit of 5% (w/w). The proposed method is a simple sample extraction and rapid HPLC analysis that could be widely used to detect intentional adulteration of lentils with common vetch.
检测用少量普通野豌豆（Vicia sativa L.）掺假的裂皮红芸豆（Lens culinaris L.）种子存在困难，因为缺乏可靠的检测方法。本研究开发了一种基于高效液相色谱-二极管阵列检测（HPLC-DAD）的分析方法，该方法使用了在普通野豌豆中发现的两种独特的化学标记物：β-氰丙氨酸（BCA）和γ-谷氨酰基-β-氰丙氨酸（GCA）。这两种标记物存在于在加拿大和塞尔维亚种植的普通野豌豆种子样本中。在加拿大、澳大利亚、美国、土耳其、叙利亚和摩洛哥种植的纯正芸豆样本中没有检测到这些化学标记物。加拿大萨斯喀彻温省芸豆加工厂出口的商业芸豆样本中也没有检测到 GCA 和 BCA。通过化学标记物可以检测到故意掺假芸豆样品中野豌豆的存在，检测限为 5%（w/w）。该方法简单，样品提取和快速 HPLC 分析可广泛用于检测故意用普通野豌豆掺假的芸豆。
