建立一种基于单克隆抗体的夹心型酶联免疫吸附试验（ELISA），用于检测食物样品中的相思豆毒素。

Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples.

机构信息

Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.

出版信息

Food Chem. 2012 Dec 15;135(4):2661-5. doi: 10.1016/j.foodchem.2012.07.047. Epub 2012 Jul 15.

DOI:10.1016/j.foodchem.2012.07.047

PMID:22980855

Abstract

Abrin is a plant toxin, which can be easily isolated from the seeds of Abrus precatorius. It may be used as a biological warfare agent. In order to detect abrin in food samples, a two-layer sandwich format enzyme-linked immunosorbent assay based on the monoclonal antibody (mAb) (as capture antibody) and rabbit polyclonal serum (as detecting antibody) was developed and applied for the determination of abrin in some food matrices. The linear range of the mAb was 1-100 μg L(-1) with a detection limit of 0.5 μg L(-1) for abrin in phosphate buffered saline (PBS). The recoveries of abrin from sausage, beer and milk samples ranged 97.5-98.6%, 95.8-98.4% and 94.8-9.6%, respectively, with a coefficient of variation (CV) of 3.7% or less. The newly developed sandwich ELISA using the mAb appears to be a reliable and useful method for detection of abrin in sausage, beer and milk.

摘要

相思豆毒素是一种植物毒素，可从相思豆的种子中轻易分离出来，也可能被用作生物战剂。为了检测食品样本中的相思豆毒素，我们开发了一种基于单克隆抗体（mAb）（作为捕获抗体）和兔多克隆血清（作为检测抗体）的双层三明治酶联免疫吸附测定法，并将其应用于一些食品基质中相思豆毒素的检测。mAb 的线性范围为 1-100μg/L(-1)，在磷酸盐缓冲盐水（PBS）中检测相思豆毒素的检出限为 0.5μg/L(-1)。从香肠、啤酒和牛奶样本中提取的相思豆毒素的回收率分别为 97.5-98.6%、95.8-98.4%和 94.8-9.6%，变异系数（CV）小于等于 3.7%。使用 mAb 建立的新的三明治 ELISA 似乎是一种可靠且有用的方法，可用于检测香肠、啤酒和牛奶中的相思豆毒素。