Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
Food Chem. 2012 Dec 15;135(4):2681-6. doi: 10.1016/j.foodchem.2012.07.053. Epub 2012 Jul 15.
Stories of recent cases about melamine misuse to raise the false impression of a high protein content of milk in China emerged in September of 2008, have become an international health event. To meet the need for rapid and reliable monitoring of melamine in milk samples, a monoclonal antibody (mAb) was produced and an inhibition enzyme-linked immunosorbent assay (ELISA) was developed based on the mAb. The standard curve was linear in the range from 0.03 to 9 ng mL(-1) with a detection limit (LOD) of 0.01 ng mL(-1). The sensitivity of the assay was 0.35 ng mL(-1). The average recovery values of melamine in the liquid milk, powder milk, dog food and cat food were 99%, 96%, 9% and 98%, respectively and the coefficient of variation (CV) values of all samples were less than 10%. The obtained results showed a potential method as a tool for the rapid and reliable monitoring of melamine in liquid milk and milk powder samples (158 words).
2008 年 9 月，中国出现了一些关于三聚氰胺被滥用以提高牛奶中蛋白质含量的虚假印象的医学案例，这已成为一个国际卫生事件。为了满足对牛奶样品中三聚氰胺进行快速可靠监测的需求，我们生产了一种单克隆抗体（mAb），并基于该 mAb 开发了抑制酶联免疫吸附测定（ELISA）。标准曲线在 0.03 至 9ng/mL 范围内呈线性，检测限（LOD）为 0.01ng/mL。该测定法的灵敏度为 0.35ng/mL。三聚氰胺在液态奶、奶粉、狗食和猫粮中的平均回收率分别为 99%、96%、9%和 98%，所有样品的变异系数（CV）值均小于 10%。结果表明，该方法有潜力成为快速可靠监测液态奶和奶粉样品中三聚氰胺的工具（158 字）。
