Department of Medicine, Division of Infectious Diseases, University of Toledo, HSC, Toledo, OH 43614, USA.
Virology. 2012 Nov 25;433(2):377-84. doi: 10.1016/j.virol.2012.08.025. Epub 2012 Sep 13.
During self-renewal of the hematopoietic stem cells there is a potential for these new cells to develop into abnormal malignant cells due to environmental, chemical or microbial challenges. This may lead to blood malignancies, including leukemia and lymphoma. Our laboratory was the first to develop a mouse model to study the development of lymphoma among pups of retrovirus infected mothers. We have established that if the newborn pups suckle breast milk from the mothers infected with a temperature sensitive Moloney Murine Leukemia Virus (MoMuLV-ts1), they develop lymphoma. Stem and progenitor cell populations were determined from blood, spleen, and thymus samples in infected mice with and without lymphoma using flow cytometry and specific protein markers, including CD117+/CD34+; CD117+/Sca-1+; CD135+/Sca-1+; Ter119+/CD71+ and CD117+/CD45+. Some stem cell populations were increased in infected mice with lymphoma. These stem cells appear to be involved in the development of lymphoma in our mouse model.
在造血干细胞的自我更新过程中，由于环境、化学或微生物的挑战，这些新细胞有可能发展成异常的恶性细胞。这可能导致血液恶性肿瘤，包括白血病和淋巴瘤。我们的实验室是第一个开发出一种小鼠模型来研究感染逆转录病毒的母亲的幼崽中淋巴瘤的发展。我们已经确定，如果新生幼崽从感染温度敏感型 Moloney 鼠白血病病毒（MoMuLV-ts1）的母亲那里吸食母乳，它们就会患上淋巴瘤。我们使用流式细胞术和特定的蛋白质标记物（包括 CD117+/CD34+；CD117+/Sca-1+；CD135+/Sca-1+；Ter119+/CD71+和 CD117+/CD45+），从感染有和没有淋巴瘤的感染小鼠的血液、脾脏和胸腺样本中确定了干细胞和祖细胞群体。一些干细胞群体在感染有淋巴瘤的小鼠中增加。这些干细胞似乎参与了我们的小鼠模型中淋巴瘤的发展。
