Department of Laboratory Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, South Korea.
J Virol Methods. 2013 Jan;187(1):79-84. doi: 10.1016/j.jviromet.2012.09.005. Epub 2012 Sep 13.
Five assays for the detection of human papillomavirus (HPV) with different assay principles were evaluated. A total of 230 cervical swab specimens were collected from subjects according to the cytologic results. All specimens were tested by the following assays: hybrid capture 2 (HC2), two real-time PCR assays (Abbott RealTime HR and AdvanSure RealTime), liquid beads microarray (GeneFinder) and peptide nucleic acid-based array (PANArray). The HPV DNA of 99 samples was sequenced to identify genotypes. Concordance rates between the results for the identification of 14 high risk HPV genotypes by any two of the evaluated assays, except for AdvanSure RealTime, ranged from 83.0% to 88.3%, and those for the identification of genotypes 16 and 18, except for HC2, were 93.0% and 96.1%, respectively. The results for the evaluation of high risk HPV genotypes by HC2 agreed with those of the other assays in 76.5-86.5% of cases. Identification of HPV genotype by GeneFinder and PANArray corresponded with that by direct sequencing in 88.9% and 84.8% of sequenced samples. This study demonstrated that HC2 and the two real-time PCR assays could be used for routine HPV screening, and the other genotyping assays can be applied for epidemiologic surveillance.
五种不同检测原理的 HPV 检测方法进行了评估。根据细胞学结果，共采集了 230 例宫颈拭子标本。所有标本均采用以下方法检测：杂交捕获 2（HC2）、两种实时 PCR 方法（Abbott RealTime HR 和 AdvanSure RealTime）、液体珠微阵列（GeneFinder）和肽核酸微阵列（PANArray）。对 99 例 HPV DNA 进行测序以鉴定基因型。除 AdvanSure RealTime 外，用两种检测方法中的任意两种检测 14 种高危 HPV 基因型的一致性率为 83.0%至 88.3%，除 HC2 外，检测 16 和 18 基因型的一致性率分别为 93.0%和 96.1%。HC2 对高危 HPV 基因型的评估结果与其他检测方法的结果在 76.5%至 86.5%的病例中一致。GeneFinder 和 PANArray 对 HPV 基因型的鉴定与直接测序的结果一致，在 88.9%和 84.8%的测序样本中一致。本研究表明，HC2 和两种实时 PCR 方法可用于常规 HPV 筛查，其他基因分型方法可用于流行病学监测。
