利用小鼠睾丸切片原位杂交技术测定精子发生过程中两个磷酸甘油酸激酶基因的转录开关。

Transcription switch of two phosphoglycerate kinase genes during spermatogenesis as determined with mouse testis sections in situ.

作者信息

Goto M, Koji T, Mizuno K, Tamaru M, Koikeda S, Nakane P K, Mori N, Masamune Y, Nakanishi Y

机构信息

Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.

出版信息

Exp Cell Res. 1990 Feb;186(2):273-8. doi: 10.1016/0014-4827(90)90306-u.

DOI:10.1016/0014-4827(90)90306-u

PMID:2298243

Abstract

In order to elucidate the mechanistic interpretations underlying differential expression of the two phosphoglycerate kinase (PGK) genes during mammalian spermatogenesis, localization of its mRNAs in mouse testis sections was determined by in situ hybridization. MRNA for nonsperm-type PGK-1 was identified in nongerminal Leydig and Sertoli cells, spermatogonia, and spermatocytes, but was not detected in spermatids. In contrast, mRNA for sperm-type PGK-2 was notable in leptotene spermatocytes, becoming most abundant in pachytene spermatocytes. It was amply present in spermatids only up to step 10, completely disappearing after step 12. It is possible to assume that a transcription switch of the two PGK genes ensued following the onset of meiosis. These findings taken together with previous observations indicate that differential expression of the two PGK genes during mammalian spermatogenesis is regulated at the transcriptional and post-transcriptional levels.

摘要

为了阐明哺乳动物精子发生过程中两个磷酸甘油酸激酶（PGK）基因差异表达背后的机制解释，通过原位杂交确定了其mRNA在小鼠睾丸切片中的定位。非精子型PGK-1的mRNA在非生殖性的莱迪希细胞和支持细胞、精原细胞以及精母细胞中被鉴定出来，但在精子细胞中未检测到。相比之下，精子型PGK-2的mRNA在细线期精母细胞中显著，在粗线期精母细胞中最为丰富。它仅在第10步之前的精子细胞中大量存在，在第12步之后完全消失。可以推测，减数分裂开始后两个PGK基因发生了转录转换。这些发现与先前的观察结果一起表明，哺乳动物精子发生过程中两个PGK基因的差异表达在转录和转录后水平受到调控。