Department of Pediatrics and Child Health Center, Albert Szent-Györgyi Clinical Center, Faculty of Medicine, University of Szeged, H-6720 Szeged, Korányi fasor 14-15, Hungary.
Life Sci. 2012 Nov 2;91(19-20):907-11. doi: 10.1016/j.lfs.2012.08.039. Epub 2012 Sep 13.
Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91(phox) subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment.
Oxidative stress parameters via photometric methods, CYBB and HMOX-1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children.
Higher blood thiobarbituric acid reactive substances (TBARS) (p<0.03) and carbonylated protein (p<0.05) levels were found in the asthmatic children than in the controls. The relative expression of CYBB was significantly lower (p<0.05) in patients treated with a low daily dose of inhaled corticosteroid (ICS), than in asthmatics not receiving ICS therapy. Higher ICS doses alone or combined with long acting β2-receptor agonists did not influence the expression significantly. No similar tendency was found as regards to HMOX-1 expression.
Elevated levels of damaged lipid (TBARS) and protein (carbonylated) products corroborate the presence of oxidative stress in the blood during bronchial asthma and suggest the presence of chronic oxidative overload. Our findings also suggest that ICS treatment can influence the relative CYBB mRNA expression in circulating leukocytes in a dose dependent manner.
在哮喘气道中观察到的氧化应激不仅局限于支气管系统。从血液中监测氧化应激标志物，特别是在儿童哮喘炎症后，将是一个很大的优势。我们的目的是测量抗氧化剂和氧化损伤生物分子的水平。我们还对自由基源 gp91(phox)亚基 (CYBB)的 NADPH 氧化酶系统和血液中的抗氧化血红素加氧酶-1 (HMOX-1)同工酶的基因表达改变感兴趣。我们的研究结果还结合了医学治疗进行了检查。
通过光度法测量 58 名哮喘儿童和 30 名健康儿童的氧化应激参数，通过实时 PCR 测量 CYBB 和 HMOX-1 的表达。
哮喘儿童的血液中硫代巴比妥酸反应性物质 (TBARS) (p<0.03) 和羰基化蛋白 (p<0.05) 水平高于对照组。接受低剂量吸入皮质类固醇 (ICS) 治疗的患者的 CYBB 相对表达明显降低 (p<0.05)，而未接受 ICS 治疗的哮喘患者则没有。单独使用或与长效β2-受体激动剂联合使用较高剂量的 ICS 并不能显著影响表达。关于 HMOX-1 的表达，没有发现类似的趋势。
受损脂质 (TBARS) 和蛋白质 (羰基化) 产物水平升高证实了支气管哮喘期间血液中存在氧化应激，并表明存在慢性氧化过载。我们的研究结果还表明，ICS 治疗可以以剂量依赖的方式影响循环白细胞中相对 CYBB mRNA 的表达。
