Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia.
FEBS Lett. 2012 Oct 19;586(20):3731-6. doi: 10.1016/j.febslet.2012.09.003. Epub 2012 Sep 13.
The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capable of a tRNA(Phe)-dependent binding to human ribosomes and cross-linking to rpS26e in the mRNA binding centre. Thus, 2'-OH groups of mRNA are unimportant for binding at the entry site but they are crucial for codon-anticodon interactions at the P site, implying the existence of mRNA-ribosome contacts that do not occur in bacteria.
使用定点交联研究了 2'-OH 基团在 mRNA 与人核糖体结合中的作用。我们发现，带有交联剂的 mRNA 和 mDNA 类似物都可以在 tRNA 存在的情况下独立于 tRNA 存在而修饰 mRNA 进入位点处的核糖体蛋白 (rps) S3e 和 S2e，但只有 mRNA 类似物能够依赖于 tRNA(Phe)与人核糖体结合并交联到 mRNA 结合中心的 rpS26e。因此，mRNA 的 2'-OH 基团对于进入位点的结合不重要，但对于 P 位的密码子-反密码子相互作用至关重要，这意味着存在不发生在细菌中的 mRNA-核糖体接触。
