Department of Anatomic Pathology, Hospital AC Camargo, São Paulo, SP, Brazil.
Am J Surg Pathol. 2012 Oct;36(10):1489-96. doi: 10.1097/PAS.0b013e3182635987.
To evaluate the reliability of novel brightfield microscopy-based dual in situ hybridization (BDISH) methods for frontline HER2 status analysis in selected suboptimally preserved breast cancer tissue samples reflecting of the worst scenario in a community.
A total of 320 morphologically poorly preserved breast invasive ductal carcinomas from the archives of 2 tertiary institutions in Brazil were selected for a tissue microarray-based analysis. 4B5 antibody was used for immunohistochemistry. Fluorescence in situ hybridization (FISH), DuoCISH, ZytoDot CISH, and silver in situ hybridization (SISH) were performed and compared. The highest agreement was observed between SISH and FISH. In addition, SISH was easier to assess in both amplified and nonamplified cases when compared with the other chromogenic methods, due to the sharpness of its dots. DuoCISH produced false-positive results, associated with thicker ill-defined dots, causing poor distinction between nonamplification and low amplification. ZytoDot CISH showed lower sensitivity, with increased frequency of false-positive results.
SISH is the most reliable of the BDISH methods, with sensitivity and specificity highly comparable with FISH. It is also less deleterious than other BDISH methods, producing signals that were more distinct and therefore more readily analyzable even in poorly preserved tissue.
评估新型亮场显微镜双重原位杂交（BDISH）方法在选择的、反映社区最差情况下的次优保存乳腺癌组织样本中进行一线 HER2 状态分析的可靠性。
从巴西 2 家 3 级医疗机构的档案中选择了 320 例形态学保存不佳的乳腺浸润性导管癌，进行基于组织微阵列的分析。使用 4B5 抗体进行免疫组织化学检测。进行了荧光原位杂交（FISH）、DuoCISH、ZytoDot CISH 和银原位杂交（SISH），并进行了比较。SISH 与 FISH 的一致性最高。此外，与其他显色方法相比，SISH 在扩增和非扩增病例中都更容易评估，因为其斑点更清晰。DuoCISH 产生了假阳性结果，与较厚的定义不明确的斑点相关，导致非扩增和低扩增之间难以区分。ZytoDot CISH 的敏感性较低，假阳性结果的频率增加。
SISH 是 BDISH 方法中最可靠的方法，其敏感性和特异性与 FISH 高度可比。它也比其他 BDISH 方法的危害性更小，产生的信号更清晰，因此即使在保存不佳的组织中也更容易进行分析。
