从牛心分离出一种新型6.8 kDa线粒体蛋白脂质及其氨基酸序列。使用快原子轰击质谱法测定分子量。

Isolation and amino acid sequence of a novel 6.8-kDa mitochondrial proteolipid from beef heart. Use of FAB-MS for molecular mass determination.

作者信息

Terzi E, Boyot P, Van Dorsselaer A, Luu B, Trifilieff E

机构信息

Laboratoire de Chimie Organique des Substances Naturelles, URA CNRS 31, Strasbourg, France.

出版信息

FEBS Lett. 1990 Jan 15;260(1):122-6. doi: 10.1016/0014-5793(90)80082-t.

DOI:10.1016/0014-5793(90)80082-t

PMID:2298292

Abstract

We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondria.

摘要

我们从牛心肌的酸性氯仿/甲醇提取物中分离出一种6.8 kDa的蛋白脂质。通过快原子轰击质谱法（FAB-MS）（m/z6834.1）测定了该多肽的分子量。其氨基酸序列部分通过直接测序确定，并通过对溴化氰和胰蛋白酶片段的表征（测序、FAB-MS和氨基酸分析）得以完善。该多肽由60个氨基酸残基组成。在兔体内产生的多克隆抗体可通过免疫印迹法将其定位在线粒体中。