Laboratory of Secretion Cell Biology, Department of Cell Biology and Genetics, State University of Maringá/UEM, Block H67, Room 19, Avenue Colombo, 5790, Maringá, PR, 87020-900, Brazil.
Endocrine. 2013 Jun;43(3):571-8. doi: 10.1007/s12020-012-9798-5. Epub 2012 Sep 16.
The goal of the present study was to investigate changes on glucose homoeostasis and of the insulin receptor (IR) and insulin receptor substrate-1 (IRS-1) signalling in pancreatic islets from MSG-obese mice submitted to or not submitted to swim training. Swim training of 90-day-old MSG mice was used to evaluate whether signalling pathways of the IR and IRS-1 in islets are involved with the insulin resistance and glucose intolerance observed in this obese animal model. The results showed that IR tyrosine phosphorylation (pIR) was reduced by 42 % in MSG-obese mice (MSG, 6.7 ± 0.2 arbitrary units (a.u.); control, 11.5 ± 0.4 a.u.); on the other hand, exercise training increased pIR by 76 % in MSG mice without affecting control mice (MSG, 11.8 ± 0.3; control, 12.8 ± 0.2 a.u.). Although the treatment with MSG increased IRS-1 tyrosine phosphorylation (pIRS-1) by 96 % (MSG, 17.02 ± 0.6; control, 8.7 ± 0.2 a.u.), exercise training also increased it in both groups (control, 13.6 ± 0.1; MSG, 22.2 ± 1.1 a.u.). Current research shows that the practice of swim training increases the tyrosine phosphorylation of IRS-1 which can modulate the effect caused by obesity in insulin receptors.
本研究的目的是探讨肥胖症模型小鼠胰岛中葡萄糖稳态和胰岛素受体（IR）及胰岛素受体底物-1（IRS-1）信号转导的变化，以及游泳训练是否对此有影响。通过对 90 日龄肥胖症模型小鼠进行游泳训练，评估胰岛中 IR 和 IRS-1 的信号通路是否与该肥胖动物模型中观察到的胰岛素抵抗和葡萄糖耐量受损有关。结果表明，肥胖症模型小鼠的 IR 酪氨酸磷酸化（pIR）降低了 42%（MSG，6.7±0.2 任意单位（a.u.）；对照组，11.5±0.4 a.u.）；另一方面，运动训练使 MSG 组的 pIR 增加了 76%，而对对照组没有影响（MSG，11.8±0.3；对照组，12.8±0.2 a.u.）。尽管肥胖症模型小鼠的 IRS-1 酪氨酸磷酸化（pIRS-1）增加了 96%（MSG，17.02±0.6；对照组，8.7±0.2 a.u.），但运动训练也使两组的 pIRS-1 都增加了（对照组，13.6±0.1；MSG，22.2±1.1 a.u.）。目前的研究表明，游泳训练可以增加 IRS-1 的酪氨酸磷酸化，从而调节肥胖对胰岛素受体的影响。
