Wang Shenlin, Shi Lichi, Okitsu Takashi, Wada Akimori, Brown Leonid S, Ladizhansky Vladimir
Department of Physics and Biophysics Interdepartmental Group, University of Guelph, Guelph, ON, Canada.
Biomol NMR Assign. 2013 Oct;7(2):253-6. doi: 10.1007/s12104-012-9421-y. Epub 2012 Sep 16.
Anabaena Sensory Rhodopsin (ASR) is a unique microbial rhodopsin that displays photocromism, interacts with soluble transducer, and may be involved in gene regulation. Here we report nearly complete spectroscopic (13)C and (15)N assignments of ASR reconstituted in lipids, obtained using two- and three-dimensional magic angle spinning solid state NMR spectroscopy on alternately (13)C labeled samples. The obtained chemical shifts are used to characterize the protein backbone conformation. They suggest that lipid-reconstituted ASR has a fold generally similar to that seen in earlier X-ray studies, but with a number of important differences. SSNMR detects double conformations for a number of residues on the cytoplasmic side.
鱼腥藻感光视紫红质（ASR）是一种独特的微生物视紫红质，具有光致变色特性，能与可溶性转导蛋白相互作用，可能参与基因调控。在此，我们报告了脂质重构的ASR几乎完整的光谱学（13）C和（15）N归属，这些归属是通过对交替（13）C标记的样品使用二维和三维魔角旋转固态核磁共振光谱法获得的。所获得的化学位移用于表征蛋白质主链构象。结果表明，脂质重构的ASR折叠结构总体上与早期X射线研究中观察到的相似，但存在一些重要差异。固态核磁共振检测到细胞质一侧许多残基存在双重构象。
