Machado Liuber Y, Dubed Marta, Díaz Héctor, Ruiz Nancy, Romay Dania, Váldes Neysi, Blanco Madeline, Silva Eladio
AIDS Research Laboratory, Carretera Tapaste y Autopista Nacional , San José de las Lajas, Mayabeque 32700, Cuba.
AIDS Res Hum Retroviruses. 2013 Feb;29(2):411-4. doi: 10.1089/AID.2012.0183. Epub 2012 Oct 25.
Knowledge of the associated mutations to transmitted drug resistance (TDR) in strains of human immunodeficiency virus type 1 (HIV-1) constitutes a fundamental premise in epidemiological surveillance. In this present study, TDR from 200 Cuban patients who were diagnosed with HIV-1 between 2009 and 2011 was analyzed. By partial reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of the HIV pol gene, an HIV subtype and transmitted resistance profile were determined. The prevalence of associated mutations to the TDR in the individuals studied was 21.5%. In the region of the reverse transcriptase, the most common mutations were K103N and M184V, while in the region of the protease they were L33F and M46L. The results of this study provide evidence of TDR in the Cuban seropositive population and suggest the necessity of making resistance assays before beginning antiretroviral therapy in HIV-1-infected patients in Cuba.
了解1型人类免疫缺陷病毒（HIV-1）毒株中与传播性耐药（TDR）相关的突变是流行病学监测的基本前提。在本研究中，分析了200名2009年至2011年间被诊断为HIV-1的古巴患者的TDR情况。通过部分逆转录酶聚合酶链反应（RT-PCR）和HIV pol基因测序，确定了HIV亚型和传播耐药谱。在所研究个体中，与TDR相关的突变患病率为21.5%。在逆转录酶区域，最常见的突变是K103N和M184V，而在蛋白酶区域则是L33F和M46L。本研究结果为古巴血清阳性人群中的TDR提供了证据，并表明在古巴对HIV-1感染患者开始抗逆转录病毒治疗之前进行耐药检测的必要性。
