Kaplan M W, Iwata R T, Sterrett C B
R. S. Dow Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, Oregon 97209-1595.
Invest Ophthalmol Vis Sci. 1990 Jan;31(1):1-8.
The effects of retinal detachment upon disk membrane assembly in rod outer segments were assessed in Xenopus laevis retinas that had been maintained in eyecup cultures for up to 4 days. In these cultures, assembly of disk membranes occurred at a normal rate in regions of the retina that remained attached to the retinal pigment epithelium. In regions of the retina that were detached from the pigment epithelium, the assembly of new disk membranes either was abnormal or was inhibited. This result cannot be attributed to reduced access of cells in the detached retina to oxygen and metabolites. The experiments described here suggest that the apposition of the retina with the pigment epithelium is a necessary condition for normal disk membrane assembly in Xenopus retinas. This effect may be mediated by contact between the rod outer segments and the pigment epithelium, or by trophic factors in the subretinal space.
在非洲爪蟾视网膜的眼杯培养物中，培养长达4天，评估视网膜脱离对视杆细胞外段盘膜组装的影响。在这些培养物中，视网膜与视网膜色素上皮保持附着的区域，盘膜以正常速率组装。在与色素上皮脱离的视网膜区域，新盘膜的组装要么异常，要么受到抑制。该结果不能归因于脱离视网膜中的细胞获取氧气和代谢物的减少。此处描述的实验表明，视网膜与色素上皮的并置是非洲爪蟾视网膜中正常盘膜组装的必要条件。这种作用可能是由视杆细胞外段与色素上皮之间的接触介导的，或者是由视网膜下间隙中的营养因子介导的。
