[Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts].

OBJECTIVE

To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1).

METHODS

Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR.

RESULTS

The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects.

CONCLUSION

Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.