Zhu Ming, Lu Feng, Gao Jianhua, Liao Yunjun
Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Sep;32(9):1265-9.
To explore the optimal seed cells derived from human adipose tissue for promoting the engraftment of transplanted adipose tissue in nude mice.
Human adipose tissue granules (0.3 ml) obtained from patients undergoing liposuction were mixed with hypoxic adipose-derived stem cells (ADCs, group A), ADCs (Group B), stromal vascular fraction (SVF) cells (group C), or pure adipose tissue granules in complete culture medium particles (group D). The mixtures were injected subcutaneously on the back of 6 nude mice, and the transplanted adipose tissues were harvested 3 months later to examine the engraftment using histological method and HE staining.
The wet weights of the adipose grafts in groups B and C (91.67∓1.472 mg and 96.67∓5.164 mg, respectively) were similar (P>0.05), but both significantly higher than those in groups A and D (61.67∓8.165 mg and 40.83 ∓4.916 mg, respectively, P<0.05). The grafts in groups A, B and Cshowed a significantly higher blood vessel density than those in group D; the blood vessel density was the highest in group C (P<0.05) and similar in groups A and B (P>0.05). Histologically, the adipose grafts in groups B and C consisted predominantly of adipose tissue, with less necrosis and fibrosis than those in groups A and D (P<0.05). The fibrosis count was the highest in group D (P<0.05), and similar in groups B and C (P>0.05).
The adipose-derived stem cells, especially ASCs and SVFs, can promote the engraftment of human adipose tissue in nude mice, indicating their potential clinical value in adipose tissue transplantation.
探索源自人脂肪组织的最佳种子细胞，以促进移植脂肪组织在裸鼠体内的植入。
将抽脂患者获取的人脂肪颗粒（0.3 ml）与低氧脂肪来源干细胞（ADCs，A组）、ADCs（B组）、基质血管成分（SVF）细胞（C组）或完全培养基颗粒中的纯脂肪组织颗粒（D组）混合。将混合物皮下注射到6只裸鼠背部，3个月后收获移植的脂肪组织，采用组织学方法和苏木精-伊红（HE）染色检查植入情况。
B组和C组脂肪移植物的湿重相似（分别为91.67±1.472 mg和96.67±5.164 mg，P>0.05），但均显著高于A组和D组（分别为61.67±8.165 mg和40.83±4.916 mg，P<0.05）。A、B和C组移植物的血管密度显著高于D组；C组血管密度最高（P<0.05），A组和B组相似（P>0.05）。组织学上，B组和C组脂肪移植物主要由脂肪组织组成，坏死和纤维化程度低于A组和D组（P<0.05）。D组纤维化计数最高（P<0.05），B组和C组相似（P>0.05）。
脂肪来源干细胞，尤其是脂肪干细胞和基质血管成分，可促进人脂肪组织在裸鼠体内的植入，表明它们在脂肪组织移植中的潜在临床价值。
