Department of Biomaterials, University of Oslo, Oslo, Norway.
Acta Biomater. 2013 Feb;9(2):5390-9. doi: 10.1016/j.actbio.2012.09.009. Epub 2012 Sep 14.
Titanium oxide (TiO₂) scaffolds have previously been reported to exhibit very low mechanical strength. However, we have been able to produce a scaffold that features a high interconnectivity, a porosity of 91% and a compressive strength above 1.2 MPa. This study analyzed the in vivo performance of the porous TiO₂ scaffolds in a peri-implant cortical defect model in the rabbit. After 8 weeks of healing, morphological microcomputed tomography analyses of the defects treated with the TiO₂ scaffolds had significantly higher bone volume, bone surface and bone surface-to-volume ratio when compared to sham, both in the cortical and bone marrow compartment. No adverse effects, i.e. tissue necrosis or inflammation as measured by lactate dehydrogenase activity and real-time reverse transcription polymerase chain reaction analysis, were observed. Moreover, the scaffold did not hinder bone growth onto the adjacent cortical titanium implant. Histology clearly demonstrated new bone formation in the cortical sections of the defects and the presence of newly formed bone in close proximity to the scaffold surface and the surface of the adjacent Ti implant. Bone-to-material contact between the newly formed bone and the scaffold was observed in the histological sections. Islets of new bone were also present in the marrow compartment albeit in small amounts. In conclusion, the present investigation demonstrates that TiO₂ scaffolds osseointegrate well and are a suitable scaffold for peri-implant bone healing and growth.
氧化钛（TiO₂）支架以前被报道具有非常低的机械强度。然而，我们已经能够生产出一种具有高连通性、91%孔隙率和抗压强度超过 1.2 MPa 的支架。本研究分析了多孔 TiO₂支架在兔种植体周围皮质缺损模型中的体内性能。在 8 周的愈合后，用 TiO₂支架治疗的缺陷的形态学微计算机断层扫描分析显示，在皮质和骨髓腔中，与假手术相比，骨体积、骨表面和骨表面积与体积比均显著更高。未观察到不良反应，即通过乳酸脱氢酶活性和实时逆转录聚合酶链反应分析测量的组织坏死或炎症。此外，支架不会阻碍相邻皮质钛植入物上的骨生长。组织学清楚地表明，在缺陷的皮质部分形成了新骨，并且在靠近支架表面和相邻 Ti 植入物表面处形成了新骨。在组织学切片中观察到新形成的骨与支架之间的骨-材料接触。骨髓腔中也存在新骨岛，尽管数量较少。总之，本研究表明 TiO₂支架具有良好的骨整合性，是一种适合种植体周围骨愈合和生长的支架。
