Institute of Plant Science, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
Bioresour Technol. 2013 May;135:622-9. doi: 10.1016/j.biortech.2012.08.068. Epub 2012 Aug 25.
The enzymatic hydrolysate of sweetpotato vines (SVH) characterized as an effective nutrients supplier with low nitrogen availability was firstly used as a substrate by Trichosporon fermentans for single cell oils (SCOs) production. Batch-fermentation experiments on various SVH based media suggested that co-fermentation of SVH and some high-sugar content substrates would be much more efficient and less-cost for SCOs production. A lipid yield of 9.6 g l(-1) with a lipid content of 35.6% was achieved on the SVH without any addition, while 27.6 and 17.7 g l(-1) lipid were respectively obtained on the fructose supplemented SVH media and the SVH mixed with acid treated wheat straw hydrolysate (WSH). The positive effect of SVH on the lipid production of T. fermentans was further demonstrated with a kinetic investigation revealing that SVH had a remarkable promoting effect on the biomass formation and the substrate uptake.
甘薯藤酶解产物（SVH）作为一种有效提供营养且氮含量低的物质，首先被出芽短梗霉用于生产单细胞油脂（SCOs）。在不同的基于 SVH 的培养基上进行分批发酵实验表明，SVH 与一些高糖含量底物的共发酵对于 SCOs 的生产将更加高效且低成本。在不添加任何物质的情况下，SVH 的脂质产量达到了 9.6 g/L，脂质含量为 35.6%，而在添加了果糖的 SVH 培养基和 SVH 与酸处理的小麦秸秆水解物（WSH）混合的培养基中，分别获得了 27.6 和 17.7 g/L 的脂质。通过动力学研究进一步证明了 SVH 对 T. fermentans 油脂生产的积极影响，该研究表明 SVH 对生物量形成和底物摄取有显著的促进作用。
