Clinical Laboratory, The Second Hospital of Shandong University, Jinan, China.
Biochimie. 2013 Mar;95(3):461-3. doi: 10.1016/j.biochi.2012.09.004. Epub 2012 Sep 14.
In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn's disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn's disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or tri-hydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.
在这项研究中，我们开发了一种快速分析肠液中胆汁酸的方法。该方法为半定量方法，所需的样本制备量很少。胆汁盐可能有助于克罗恩病的发病机制。在一项初步研究中，我们通过分析来自七名克罗恩病患者和三名健康对照者的回肠液中的胆汁酸含量来证明该方法。观察到的主要胆汁酸为二羟和/或三羟胆烷酸、二羟和/或三羟胆酰甘氨酸、二羟和/或三羟胆酰牛磺酸、单硫酸化二羟胆烷酸和单硫酸化二羟胆酰甘氨酸。该方法可类似地应用于源自肠内其他部位的样本。
