Laboratorio de Neurobiología de la Memoria, IFIByNE-CONICET, Departamento de Fisiología Biología Molecular y Celular, Universidad de Buenos Aires, Buenos Aires, Argentina.
Arthropod Struct Dev. 2013 Jan;42(1):17-25. doi: 10.1016/j.asd.2012.09.002. Epub 2012 Sep 14.
The aim of the present work is to provide an anatomical description of the cardiac system in the crab Neohelice granulata and evidence of the presence of GABA by means of immunohistochemistry. The ganglionic trunk was found lying on the inner surface of the heart's dorsal wall. After dissection, this structure appeared as a Y-shaped figure with its major axis perpendicular to the major axis of the heart. Inside the cardiac ganglion, we identified four large neurons of 63.7 μm ± 3.7 in maximum diameter, which were similar to the motor neurons described in other decapods. All the GABA-like immunoreactivity (GABAi) was observed as processes entering mainly the ganglionic trunk and branching in slender varicose fibers, forming a network around the large neurons suggesting that GABAi processes contact them. Our findings strengthen previous results suggesting that the GABAergic system mediates the cardio-inhibitory response upon sensory stimulation.
本研究旨在提供蟹 Neohelice granulata 心脏系统的解剖描述，并通过免疫组织化学方法证明 GABA 的存在。神经节干位于心脏背壁的内表面。解剖后，该结构呈 Y 形，其主轴垂直于心脏的主轴。在心神经节内，我们鉴定出四个最大直径为 63.7μm±3.7 的大型神经元，它们与其他十足目动物的运动神经元相似。所有 GABA 样免疫反应（GABAi）均表现为进入神经节干的过程，并以细长的曲张纤维分支，形成围绕大型神经元的网络，表明 GABAi 过程与它们接触。我们的发现加强了先前的结果，表明 GABA 能系统介导感觉刺激引起的心脏抑制反应。
