Department of Pharmacognosy, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
J Alzheimers Dis. 2013;33(1):217-29. doi: 10.3233/JAD-2012-120841.
Triptolide, a biologically active natural product from Tripterygium wilfordii, protects neurons from inflammation-mediated damage. Our results showed for the first time that triptolide inhibited the expression of CXCR2 and presenilin in a neuroblastoma cell line SHSY5Ysw. Moreover, triptolide potently inhibited amyloid-β1-42 production with IC50 value of 30 pM in HEK293sw cells or 2 nM in SHSY5Ysw cells, respectively. We also demonstrated that triptolide prevented primary cortical neurons from chemokine CXCL1-induced cytotoxicity. Therefore, our study indicates that the neural protective effect of triptolide is largely mediated by inhibiting CXCR2 activity.
雷公藤红素是从雷公藤中提取的一种具有生物活性的天然产物，可保护神经元免受炎症介导的损伤。我们的结果首次表明，雷公藤红素可抑制神经母细胞瘤细胞系 SHSY5Ysw 中 CXCR2 和早老素的表达。此外，雷公藤红素分别在 HEK293sw 细胞中 IC50 值为 30 pM 和在 SHSY5Ysw 细胞中 IC50 值为 2 nM 时，强烈抑制淀粉样蛋白-β1-42 的产生。我们还表明，雷公藤红素可防止原代皮质神经元受到趋化因子 CXCL1 诱导的细胞毒性。因此，我们的研究表明，雷公藤红素的神经保护作用主要是通过抑制 CXCR2 活性来介导的。
