Academic Unit of Respiratory Medicine, Department of Infection and Immunity, University of Sheffield, UK.
Cell Tissue Res. 2012 Dec;350(3):455-64. doi: 10.1007/s00441-012-1490-9. Epub 2012 Sep 18.
Despite being initially identified in mice, little is known about the sites of production of members of the BPI fold (BPIF) containing (PLUNC) family of putative innate defence proteins in this species. These proteins have largely been considered to be specificaly expressed in the respiratory tract, and we have recently shown that they exhibit differential expression in the epithelium of the proximal airways. In this study, we have used species-specific antibodies to systematically localize two members of this protein family; BPIFA1 (PLUNC/SPLUNC1) and BPIFB1 (LPLUNC1) in adult mice. In general, these proteins exhibit distinct and only partially overlapping localization. BPIFA1 is highly expressed in the respiratory epithelium and Bowman's glands of the nasal passages, whereas BPIFB1 is present in small subset of goblet cells in the nasal passage and pharynx. BPIFB1 is also present in the serous glands in the proximal tongue where is co-localised with the salivary gland specific family member, BPIFA2E (parotid secretory protein) and also in glands of the soft palate. Both proteins exhibit limited expression outside of these regions. These results are consistent with the localization of the proteins seen in man. Knowledge of the complex expression patterns of BPIF proteins in these regions will allow the use of tractable mouse models of disease to dissect their function.
尽管最初在小鼠中被鉴定出来，但对于该物种中含 BPI 折叠（BPIF）的成员（PLUNC）家族的先天防御蛋白的产生部位知之甚少。这些蛋白质在很大程度上被认为是专门在呼吸道中表达的，我们最近表明它们在近端气道的上皮细胞中表现出不同的表达。在这项研究中，我们使用了种特异性抗体来系统地定位该蛋白家族的两个成员；BPIFA1（PLUNC/SPLUNC1）和 BPIFB1（LPLUNC1）在成年小鼠中。一般来说，这些蛋白质表现出不同的、只有部分重叠的定位。BPIFA1 在鼻道的呼吸道上皮和 Bowman 腺中高度表达，而 BPIFB1 存在于鼻道和咽的一小部分杯状细胞中。BPIFB1 也存在于舌的近端舌头上的浆液腺中，与唾液腺特异性家族成员 BPIFA2E（腮腺分泌蛋白）共存，也存在于软腭的腺体中。这两种蛋白质在这些区域之外的表达都很有限。这些结果与在人类中观察到的蛋白质定位一致。了解这些区域中 BPIF 蛋白的复杂表达模式将允许使用可处理的疾病小鼠模型来剖析它们的功能。
