Alves Daniel Berretta Moreira, Bingle Lynne, Bingle Colin David, Lourenço Silvia Vanessa, Silva Andréia Aparecida, Pereira Débora Lima, Vargas Pablo Agustin
Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Department of Oral Diagnosis, Piracicaba, SP, Brazil.
University of Sheffield, School of Clinical Dentistry, Academic Unit of Oral and Maxillofacial Pathology, Sheffield, UK.
Braz Oral Res. 2017 Jan 16;31:e6. doi: 10.1590/1807-3107BOR-2017.vol31.0006.
The aim of this study was to determine expression, not previously described, of PLUNC (palate, lung, and nasal epithelium clone) (BPI-fold containing) proteins in major and minor salivary glands from very early fetal tissue to the end of the second trimester and thus gain further insight into the function of these proteins. Early fetal heads, and major and minor salivary glands were collected retrospectively and glands were classified according to morphodifferentiation stage. Expression of BPI-fold containing proteins was localized through immunohistochemistry. BPIFA2, the major BPI-fold containing protein in adult salivary glands, was detected only in the laryngeal pharynx; the lack of staining in salivary glands suggested salivary expression is either very late in development or is only in adult tissues. Early expression of BPIFA1 was seen in the trachea and nasal cavity with salivary gland expression only seen in late morphodifferentiation stages. BPIFB1 was seen in early neural tissue and at later stages in submandibular and sublingual glands. BPIFA1 is significantly expressed in early fetal oral tissue but BPIFB1 has extremely limited expression and the major salivary BPIF protein (BPIFA2) is not produced in fetal development. Further studies, with more sensitive techniques, will confirm the expression pattern and enable a better understanding of embryonic BPIF protein function.
本研究的目的是确定PLUNC（腭、肺和鼻上皮克隆）（含BPI结构域）蛋白在极早期胎儿组织至妊娠中期结束时的大、小唾液腺中的表达情况（此前未描述过），从而进一步深入了解这些蛋白的功能。回顾性收集早期胎儿头部以及大、小唾液腺，并根据形态分化阶段对腺体进行分类。通过免疫组织化学对含BPI结构域蛋白的表达进行定位。成人唾液腺中主要的含BPI结构域蛋白BPIFA2仅在喉咽部被检测到；唾液腺中缺乏染色表明唾液表达要么在发育后期才出现，要么仅存在于成人组织中。BPIFA1在气管和鼻腔中早期表达，唾液腺表达仅在形态分化后期出现。BPIFB1在早期神经组织中可见，在后期下颌下腺和舌下腺中也可见。BPIFA1在早期胎儿口腔组织中显著表达，但BPIFB1表达极其有限，且主要的唾液BPIF蛋白（BPIFA2）在胎儿发育过程中不产生。采用更灵敏技术的进一步研究将证实表达模式，并有助于更好地理解胚胎BPIF蛋白的功能。
