Beijing National Laboratory of Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beiyijie, PR China.
Chemistry. 2012 Oct 22;18(43):13755-61. doi: 10.1002/chem.201201993. Epub 2012 Sep 17.
The development of magnetic nanoparticles with multiple functions has been an ever-growing field because of their diverse applications in drug delivery, biosensing, cell labeling, and so on. In this study, a facile method was developed to construct multifunctional magnetic nanocomposites. The approach is based on the use of poly(glycidyl methacrylate), PGMA, with numerous epoxy groups as reactive polymer to combine with fluorescent dye, the surface of magnetic nanoparticles, and targeting ligands directly without expatiatory functionality design. The resultant nanocomposites with good superparamagnetic and fluorescent properties could be exploited for bioimaging. Moreover, after conjugation with a model protein, namely, transferrin, which specifically targets cells overexpressing transferrin receptors, the nanocomposites could be used selectively to recognize Hela cells in comparison with nonconjugated ones. These results indicate that the newly designed magnetic nanocomposites with PGMA as functional polymer could serve as a novel versatile platform to conjugate with various molecules for construction of diverse multifunctional magnetic nanocomposites to meet different requirements and potential uses in nanomedicine and biological chemistry.
多功能磁性纳米粒子的发展一直是一个不断增长的领域，因为它们在药物输送、生物传感、细胞标记等方面有多种应用。在这项研究中，开发了一种简便的方法来构建多功能磁性纳米复合材料。该方法基于使用具有多个环氧基团的聚(甲基丙烯酸缩水甘油酯)（PGMA）作为反应性聚合物，直接与荧光染料、磁性纳米粒子的表面和靶向配体结合，而无需进行繁琐的功能设计。所得的纳米复合材料具有良好的超顺磁性和荧光性能，可用于生物成像。此外，在与模型蛋白（即转铁蛋白）缀合后，该纳米复合材料可以特异性地识别过度表达转铁蛋白受体的 Hela 细胞，与未缀合的纳米复合材料相比，具有更好的选择性。这些结果表明，以 PGMA 为功能聚合物的新型磁性纳米复合材料可以作为一种新型的通用平台，与各种分子结合，构建不同的多功能磁性纳米复合材料，以满足纳米医学和生物化学领域不同的需求和潜在用途。
