Department of Chemistry, Fujita Health University School of Medicine, Toyoake, Japan.
Cell Biochem Funct. 2013 Apr;31(3):254-62. doi: 10.1002/cbf.2895. Epub 2012 Sep 18.
We examined whether water-immersion restraint stress (WIRS) disrupts nonenzymatic antioxidant defense systems through ascorbic acid depletion in the adrenal gland of rats. Rats were exposed to WIRS for 0.5, 1.5, 3 or 6 h. WIRS increased serum adrenocorticotropic hormone, corticosterone and glucose concentrations and adrenal corticosterone content at each time point. WIRS increased adrenal lipid peroxide content at 3 and 6 h, and the increase was twofold higher than the unstressed level at 6 h. WIRS decreased adrenal ascorbic acid content at each time point, and the decrease reached one-third of the unstressed level at 6 h. WIRS increased adrenal reduced glutathione content at 0.5 and 6 h but reduced that content to half of the unstressed level at 6 h. WIRS increased adrenal α-tocopherol content at 1.5 h but returned that content to the unstressed level thereafter. When rats with 6 h of WIRS was orally preadministered with l-ascorbic acid (250 mg/kg), WIRS-induced changes in adrenal lipid peroxide, ascorbic acid and reduced glutathione contents were attenuated without any change in stress response. These results indicate that WIRS disrupts nonenzymatic antioxidant defense systems through rapid and continuous ascorbic acid depletion in the adrenal gland of rats.
我们研究了浸水束缚应激（WIRS）是否通过大鼠肾上腺中抗坏血酸耗竭来破坏非酶抗氧化防御系统。大鼠暴露于 WIRS 0.5、1.5、3 或 6 h。WIRS 增加了血清促肾上腺皮质激素、皮质酮和葡萄糖浓度以及每个时间点的肾上腺皮质酮含量。WIRS 在 3 和 6 h 增加了肾上腺脂质过氧化物含量，并且在 6 h 时增加了两倍以上。WIRS 在每个时间点降低了肾上腺抗坏血酸含量，并且在 6 h 时降低到未应激水平的三分之一。WIRS 在 0.5 和 6 h 增加了肾上腺还原型谷胱甘肽含量，但在 6 h 将其含量降低到未应激水平的一半。WIRS 在 1.5 h 增加了肾上腺 α-生育酚含量，但此后又恢复到未应激水平。当大鼠接受 6 h 的 WIRS 并口服预先给予 L-抗坏血酸（250 mg/kg）时，WIRS 诱导的肾上腺脂质过氧化物、抗坏血酸和还原型谷胱甘肽含量的变化得到减弱，而应激反应没有任何变化。这些结果表明，WIRS 通过大鼠肾上腺中抗坏血酸的快速和持续耗竭破坏非酶抗氧化防御系统。
