用于大量植物样本的自动化DNA提取

Automated DNA extraction for large numbers of plant samples.

作者信息

Mehle Nataša, Nikolić Petra, Rupar Matevž, Boben Jana, Ravnikar Maja, Dermastia Marina

机构信息

National Institute of Biology, Ljubljana, Slovenia.

出版信息

Methods Mol Biol. 2013;938:139-45. doi: 10.1007/978-1-62703-089-2_12.

DOI:10.1007/978-1-62703-089-2_12

PMID:22987412

Abstract

The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.

摘要

本文所述方法是一种快速的全DNA提取程序，适用于大量需要进行病原体检测的植物样本。该程序将粗提物简单快速的匀浆步骤与基于DNA与磁珠结合的DNA提取相结合。DNA在一个自动化过程中得以纯化，在此过程中磁珠会经过一系列洗涤缓冲液。洗脱的DNA适用于PCR反应中的高效扩增。