植原体膜重组蛋白的制备。
Preparation of phytoplasma membrane recombinant proteins.
作者信息
Galetto Luciana, Siampour Majid, Marzachì Cristina
机构信息
Istituto di Virologia Vegetale, CNR, Torino, Italy.
出版信息
Methods Mol Biol. 2013;938:351-69. doi: 10.1007/978-1-62703-089-2_30.
PMID:22987430
Abstract
The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol for the heterologous expression of phytoplasma membrane proteins in Escherichia coli is described, together with a procedure to purify a suitable quantity of fusion antigen for application in the study of phytoplasma-host interactions.
摘要
研究植原体膜蛋白与宿主细胞蛋白的相互作用对于理解这些不可培养微生物在宿主内的生命周期至关重要。本文描述了在大肠杆菌中异源表达植原体膜蛋白的逐步方案，以及纯化适量融合抗原以用于研究植原体与宿主相互作用的程序。
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