Haplotypic association of ADAM33 (T+1, S+1 and V - 3) gene variants in genetic susceptibility to asthma in Indian population.

AIM

The aim of this study was to find out if the ADAM33 gene polymorphisms T+1(A>G), S+1(T>A) and V - 3(C>T) and their haplotypes play any role in genetic susceptibility to asthma.

SUBJECTS AND METHODS

Three hundred and ninety healthy controls and 386 asthmatic patients from Lucknow, India, were recruited for the study. Subjects were aged between 1-50 years. Among total recruited asthma cases, 95 (24.6%) had mild intermittent asthma, 235 (60.9%) had mild persistent asthma and 56 (14.5%) had moderate persistent asthma. Genotyping was carried out using the Polymerase Chain Reaction and Restriction Fragment Length polymorphism (PCRRFLP) method.

RESULTS

No significant differences in the genotype or allele frequencies of ADAM33 gene polymorphisms between asthmatic patients and healthy controls were found [p-value>0.05 (All the p-values were Bonferroni corrected)]. Also, no association of studied SNPs with the severity of the disease asthma was observed. However, the TTA haplotype was observed to be associated with asthma (OR = 3.4; 95%CI = 1.4-8.7; p = 0.002).

CONCLUSIONS

SNPs, T+1, S+1 and V - 3 do not individually confer any significant risk of asthma or its severity, but haplotype analysis suggests all three polymorphisms together play an important role in the disease of asthma.