Department of Center Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
Bioresour Technol. 2012 Nov;124:245-51. doi: 10.1016/j.biortech.2012.05.145. Epub 2012 Jul 10.
An exopolysaccharide (EPS) with a molecular weight of 230 kDa, was isolated from Bordetella sp. B4. The EPS was identified as linear alpha-1,6-(6-methyl)-glucan with N-acetyl-D-glucosamine branches at alpha-1, 4-linkages by IR and NMR spectroscopy. The free radical scavenging capacities of EPS on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)), H(2)O(2), -OH and lipid peroxidation were 2-, 86-, 134- and 18-fold higher than that of ascorbic acid, respectively. Compared with ascorbic acid, the EPS was more effective in preventing DNA and protein from free radical damage induced by 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH). More significantly, the EPS did not degrade DNA and protein by the pro-oxidant effect in the presence of copper ions and H(2)O(2). Furthermore, EPS could protect human umbilical vein endothelium cells (HUVECs) from high glucose-mediated damage. The production of EPS reached 10.2 g/L in the fermentation medium containing 3.0 g/L cholesterol, suggesting that Bordetella sp. B4 was a potential producer of antioxidant EPS.
从博德特氏菌 B4 中分离出一种分子量为 230 kDa 的胞外多糖（EPS）。通过红外和核磁共振光谱鉴定，EPS 是一种线性α-1,6-(6-甲基)-葡聚糖，具有α-1,4-连接的 N-乙酰-D-葡萄糖胺支链。EPS 对 2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS(+))、H(2)O(2)、-OH 和脂质过氧化的自由基清除能力分别比抗坏血酸高 2、86、134 和 18 倍。与抗坏血酸相比，EPS 更有效地防止 2,2'-偶氮双（2-脒基丙烷）二盐酸盐（AAPH）诱导的 DNA 和蛋白质免受自由基损伤。更重要的是，EPS 在铜离子和 H(2)O(2)存在下没有通过促氧化剂作用降解 DNA 和蛋白质。此外，EPS 可以保护人脐静脉内皮细胞（HUVECs）免受高葡萄糖介导的损伤。在含有 3.0 g/L 胆固醇的发酵培养基中，EPS 的产量达到 10.2 g/L，表明博德特氏菌 B4 是一种潜在的抗氧化 EPS 产生菌。
