CSIR - National Botanical Research Institute, Rana Pratap Marg, Lucknow, UP 226001, India.
Res Microbiol. 2012 Sep-Oct;163(8):511-7. doi: 10.1016/j.resmic.2012.08.004. Epub 2012 Sep 7.
The development of genetically modified plants for agriculture has provided numerous economic benefits, but has also raised concern over the potential impact of transgenic plants upon the environment. The rhizosphere is the soil compartment that is directly under the influence of living roots; it constitutes a complex niche likely to be exploited by a wide variety of bacteria potentially influenced by the introduction of transgenes in genetically modified plants. In the present study, the impact of overexpression of the salinity stress-tolerant minichromosome maintenance complex subunit 6 (MCM6) gene upon functional diversity and soil enzymatic activity in the rhizosphere of transgenic tobacco in the presence and absence of salt stress was examined. The diversity of culturable bacterial communities and soil enzymes, namely, dehydrogenases and acid phosphatases, was assessed and revealed no significant (or only minor) alterations due to transgenes in the rhizosphere soil of tobacco plants. Patterns in principal components analysis showed clustering of transgenic and non-transgenic tobacco plants according to the fingerprint of their associated bacterial communities. However, the presence of MCM6 tobacco did not cause changes in microbial populations, soil enzymatic activities or the functional diversity of the rhizosphere soil microbial community.
农业转基因植物的发展带来了众多经济效益，但也引发了人们对转基因植物对环境潜在影响的担忧。根际是受活根直接影响的土壤部分；它构成了一个复杂的小生境，可能会被各种可能受转基因植物中转基因影响的细菌利用。在本研究中，研究了耐盐胁迫的微小染色体维持复合物亚基 6（MCM6）基因过表达对盐胁迫存在和不存在条件下转基因烟草根际功能多样性和土壤酶活性的影响。评估了可培养细菌群落和土壤酶（脱氢酶和酸性磷酸酶）的多样性，结果表明，由于根际土壤中转基因的存在，并没有导致烟草根际土壤中细菌群落发生显著（或仅轻微）变化。主成分分析的模式表明，根据其相关细菌群落的指纹，转基因和非转基因烟草植物聚类。然而，MCM6 烟草的存在并没有引起微生物种群、土壤酶活性或根际土壤微生物群落功能多样性的变化。
