Sanchez Cecilia P, Pfahler Judith, Del Portillo Hernando A, Lanzer Michael
Department of Infectious Diseases, Universitätsklinikum Heidelberg, Heidelberg, Germany.
Methods Mol Biol. 2013;923:151-9. doi: 10.1007/978-1-62703-026-7_10.
Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating interactions with the human host still awaits further technological breakthroughs, foremost the establishment of a continuous in vitro culture system. Nevertheless, the first steps towards domesticating P. vivax for research purposes have been made. Transfection will eventually help to better understand the unique pathogenic features displayed by P. vivax, such as the host cell specificity for reticulocytes and the occurrence of relapses. Transfection will also be an invaluable tool for studies related to the emerging drug resistance, and it will help identify and validate novel targets for rational intervention.
在此，我们描述了间日疟原虫瞬时转染的方法。对间日疟原虫进行基因操作的能力使这种重要的人类疟原虫更易于进行分子研究。然而，对这种寄生虫及其与人类宿主的致病介导相互作用进行系统分析仍有待进一步的技术突破，首要的是建立连续的体外培养系统。尽管如此，已经朝着为研究目的驯化间日疟原虫迈出了第一步。转染最终将有助于更好地理解间日疟原虫所表现出的独特致病特征，例如对网织红细胞的宿主细胞特异性以及复发的发生。转染对于与新出现的耐药性相关的研究也将是一种非常有价值的工具，并且将有助于识别和验证合理干预的新靶点。
