Li Ang, Rénia Laurent, Lim Chwee Teck, Russell Bruce
Singapore-MIT Alliance for Research and Technology, Singapore, Singapore.
Methods Mol Biol. 2013;923:299-305. doi: 10.1007/978-1-62703-026-7_21.
Atomic Force Microscopy (AFM) is a powerful tool for exploring the interaction between ligands and receptors, as well as their exact locations on the red cell surface. Here we discuss current and future applications for AFM based single-molecule force spectroscopy to study adhesion of Plasmodium-infected red blood cells. A protocol is provided for simultaneous topography and recognition imaging of the surface of Plasmodium falciparum-infected cells using CD36 functionalized tips.
原子力显微镜（AFM）是探索配体与受体之间相互作用及其在红细胞表面确切位置的有力工具。在此，我们讨论基于AFM的单分子力谱在研究疟原虫感染红细胞黏附方面的当前及未来应用。本文提供了一种使用CD36功能化探针同时对恶性疟原虫感染细胞表面进行形貌和识别成像的方案。
