肝细胞内肝期发育及裂殖子释放的分析。

Analysis of liver stage development in and merozoite release from hepatocytes.

作者信息

Stanway Rebecca R, Schmuckli-Maurer Jacqueline, Heussler Volker T

机构信息

Institute of Cell Biology, University of Bern, Bern, Switzerland.

出版信息

Methods Mol Biol. 2013;923:411-27. doi: 10.1007/978-1-62703-026-7_29.

DOI:10.1007/978-1-62703-026-7_29

PMID:22990795

Abstract

Exoerythrocytic Plasmodium parasites infect hepatocytes and develop to huge multinucleated schizonts inside a parasitophorous vacuole. Finally, thousands of merozoites are formed and released into the host cell cytoplasm by complete disintegration of the parasitophorous vacuole membrane. This, in turn, results in death and detachment of the infected hepatocyte, followed by the formation of merosomes. The fast growth of the parasite and host cell detachment are hallmarks of liver stage development and can easily be monitored. Here, we describe how to translate these observations into assays for characterizing parasite development. Additionally, other recently introduced techniques and tools to analyze and manipulate liver stage parasites are also discussed.

摘要

疟原虫的红细胞外期寄生虫感染肝细胞，并在寄生泡内发育成巨大的多核裂殖体。最后，通过寄生泡膜的完全解体形成数千个裂殖子并释放到宿主细胞质中。这反过来又导致被感染肝细胞的死亡和脱离，随后形成裂殖子体。寄生虫的快速生长和宿主细胞脱离是肝期发育的标志，并且很容易监测。在这里，我们描述了如何将这些观察结果转化为用于表征寄生虫发育的分析方法。此外，还讨论了其他最近引入的用于分析和操纵肝期寄生虫的技术和工具。