College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Biochem Genet. 2012 Dec;50(11-12):936-45. doi: 10.1007/s10528-012-9533-1. Epub 2012 Sep 19.
The crested ibis, one of the most endangered birds in the world, could benefit from research into its genetic diversity as a tool for conservation in the future. Tyrosinase is thought to play a major role in the production of common yellow to black melanins in birds. We have cloned and sequenced four exons of the crested ibis tyrosinase gene and discovered that the amino acid sequence has high similarity to zebra finch tyrosinase (93 %), followed by chicken (91 %) and quail (91 %). Some functional and structural domains in the crested ibis tyrosinase coding area were found to be conserved during evolution. Nine sequence variants were found in the partial coding sequence, one in exon 1 and eight in exon 4. Sequence variant 1 (SV1) shows intermediate polymorphism (0.25 < PIC < 0.5), and further study is needed to determine whether it can be used as a potential molecular marker in crested ibis artificial breeding programs.
朱鹮是世界上最濒危的鸟类之一，对其遗传多样性的研究可以为未来的保护提供帮助。酪氨酸酶被认为在鸟类中常见的黄色到黑色黑素的生成中起着重要作用。我们已经克隆和测序了朱鹮酪氨酸酶的四个外显子，并发现其氨基酸序列与斑胸草雀酪氨酸酶（93%）高度相似，其次是鸡（91%）和鹌鹑（91%）。在进化过程中，发现朱鹮酪氨酸酶编码区的一些功能和结构域是保守的。在部分编码序列中发现了 9 个序列变异体，1 个在 exon 1 中，8 个在 exon 4 中。序列变异体 1（SV1）表现出中等多态性（0.25<PIC<0.5），需要进一步研究以确定它是否可以作为朱鹮人工繁殖计划中的潜在分子标记。
