Wong Christopher C, Curthoys Ian S, O'Leary Stephen J, Jones Allan S
Australian Centre for Microscopy and Microanalysis, University of Sydney, Sydney, Australia.
Acta Otolaryngol. 2013 Jan;133(1):22-7. doi: 10.3109/00016489.2012.715751. Epub 2012 Sep 19.
The use of both gadolinium chloride (GdCl(3)) and osmium tetroxide (OsO(4)) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl(3) in radiological assessments and OsO(4) in more detailed anatomical studies and pathways of labyrinthine pathogenesis using X-ray microtomography (microCT).
To assess an improved OsO(4) staining protocol and compare the staining affinities against GdCl(3).
Guinea pig temporal bones were stained with either GdCl(3) (2% w/v) for 7 days or OsO(4) (2% w/v) for 3 days, and scanned in a microCT system. The post-scanned datasets were then assessed in a 3D rendering program.
The enhanced soft tissue contrast as presented in the temporal bones stained with either GdCl(3) or OsO(4) allowed for the membranous labyrinth to be visualized throughout the whole specimen. GdCl(3)-stained specimens presented more defined contours of the bone profile in the radiographs, while OsO(4)-stained specimens provided more anatomical detail of individual intralabyrinthine structures, hence allowing spatial relationships to be visualized with ease in a 3D rendering context and 2D axial slice images.
与对照样本相比，使用氯化钆（GdCl₃）和四氧化锇（OsO₄）均可使膜迷路及其他迷路内结构以不同强度显影。这一初步比较显示了GdCl₃在放射学评估中的优势以及OsO₄在使用X射线显微断层扫描（microCT）进行更详细的解剖学研究和迷路发病机制途径研究中的优势。
评估改进的OsO₄染色方案，并比较其与GdCl₃的染色亲和力。
豚鼠颞骨分别用2%（w/v）的GdCl₃染色7天或2%（w/v）的OsO₄染色3天，然后在microCT系统中进行扫描。扫描后的数据集随后在三维渲染程序中进行评估。
用GdCl₃或OsO₄染色的颞骨中软组织对比度增强，使得整个标本中的膜迷路均可显影。GdCl₃染色的标本在X线照片中呈现出更清晰的骨轮廓，而OsO₄染色的标本提供了单个迷路内结构更详细的解剖细节，从而便于在三维渲染环境和二维轴向切片图像中轻松观察空间关系。
