评估精子获能和顶体反应的方法，不包括精子穿透试验。
Methods for the assessment of sperm capacitation and acrosome reaction excluding the sperm penetration assay.
作者信息
De Jonge Christopher J, Barratt Christopher L R
机构信息
Department of Obstetrics, Gynecology & Women's Health, University of Minnesota Physicians Reproductive Medicine Center, Minneapolis, MN, USA.
出版信息
Methods Mol Biol. 2013;927:113-8. doi: 10.1007/978-1-62703-038-0_11.
Assessing the ability of human spermatozoa to acquire fertilizing potential (capacitation) by stimulating exocytosis of the contents of the acrosome (acrosome reaction) is thought to have diagnostic potential (De Jonge, Reprod Med Rev 3:159-178, 1994). Calcium-mobilizing agents, such as calcium ionophores (A23187) and progesterone, stimulate the acrosome reaction in vitro (Brucker and Lipford, Hum Reprod Update 1:51-62, 1995). Acrosomal status is easily detected using Pisum sativum Agglutinin labeled with fluorescein isothiocyanate (Cross and Meizel, Biol Reprod 41:635-641, 1989). Herein we describe a procedure for assessing capacitation and the acrosome reaction of human spermatozoa in vitro.
通过刺激顶体内容物的胞吐作用（顶体反应）来评估人类精子获得受精潜能（获能）的能力被认为具有诊断潜力（德容格，《生殖医学评论》3:159 - 178，1994年）。钙动员剂，如钙离子载体（A23187）和孕酮，在体外刺激顶体反应（布鲁克和利普福德，《人类生殖更新》1:51 - 62，1995年）。使用异硫氰酸荧光素标记的豌豆凝集素可以很容易地检测顶体状态（克罗斯和梅泽尔，《生物生殖》41:635 - 641，1989年）。在此，我们描述一种体外评估人类精子获能和顶体反应的方法。
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