1Department of Histology and Embryology, Akdeniz University School of Medicine, Antalya, Turkey.
Reprod Sci. 2014 May;21(5):573-81. doi: 10.1177/1933719113504467. Epub 2013 Sep 27.
In mammalian species, acquisition of sperm fertilization competence is dependent on the phenomenon of sperm capacitation. One of the key elements of capacitation is protein tyrosine phosphorylation (TP) in various sperm membrane regions. In previous studies performed, the pattern of TP was examined in human sperm bound to zona pellucida of oocytes. In the present comparative study, TP patterns upon sperm binding to the zona pellucida or hyaluronic acid (HA) were investigated in spermatozoa arising from the same semen samples. Tyrosine phosphorylation, visualized by immunofluorescence, was localized within the acrosomal cap, equatorial head region, neck, and the principal piece. Tyrosine phosphorylation has increased in a time-related manner as capacitation progressed, and the phosphorylation pattern was identical within the principal piece and neck, regardless of the sperm bound to the zona pellucida or HA. Thus, the data demonstrated that the patterns of sperm activation-related TP were similar regardless of the spermatozoa bound to zona pellucida or HA. Further, sperm with incomplete development, as detected by excess cytoplasmic retention, failed to exhibit TP.
在哺乳动物物种中，精子获得受精能力取决于精子获能现象。获能的一个关键因素是各种精子膜区域的蛋白质酪氨酸磷酸化 (TP)。在之前进行的研究中，检查了与人卵透明带结合的精子中的 TP 模式。在本比较研究中，研究了来自同一精液样本的精子与透明带或透明质酸 (HA) 结合时的 TP 模式。通过免疫荧光观察到的酪氨酸磷酸化定位于顶体帽、赤道头部区域、颈部和主段。随着获能的进行，酪氨酸磷酸化呈时间相关的方式增加，并且无论与透明带还是 HA 结合的精子，其磷酸化模式在主段和颈部都是相同的。因此，数据表明，与透明带或 HA 结合的精子无关，精子激活相关的 TP 模式相似。此外，通过细胞质滞留检测到的发育不完全的精子未能表现出 TP。
