Rodriguez-Sosa Jose R, Tang Lin, Dobrinski Ina
Department of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2013;927:489-500. doi: 10.1007/978-1-62703-038-0_42.
Testicular tissue grafting and male germ cell transplantation are techniques that offer unprecedented opportunities to study testicular function and development. While testicular tissue grafting allows recapitulation of testis development and spermatogenesis from immature males of different mammalian species in recipient mice, germ cell transplantation results in donor-derived spermatogenesis in recipient testes.Testicular tissue grafting results in spermatogenesis from a wide variety of large animal donor species and is therefore an attractive way to study testis development and spermatogenesis and preserve fertility of immature males. Germ cell transplantation represents a functional reconstitution assay for identification of spermatogonial stem cells (SSCs) in a given donor cell population and serves as a valuable tool to study stem cell biology and spermatogenesis. In this chapter we provide detailed methodology to successfully perform both techniques.
睾丸组织移植和雄性生殖细胞移植是为研究睾丸功能和发育提供了前所未有的机会的技术。睾丸组织移植能够在受体小鼠中重现来自不同哺乳动物物种未成熟雄性的睾丸发育和精子发生过程，而生殖细胞移植则会在受体睾丸中产生供体来源的精子发生。睾丸组织移植能使多种大型动物供体物种产生精子发生，因此是研究睾丸发育和精子发生以及保存未成熟雄性生育能力的一种有吸引力的方法。生殖细胞移植是一种用于鉴定给定供体细胞群体中精原干细胞（SSCs）的功能重建检测方法，也是研究干细胞生物学和精子发生的宝贵工具。在本章中，我们提供了成功实施这两种技术的详细方法。
