质谱法检测法国两起 A 型肉毒中毒爆发期间患者血清中的毒素。
Toxin detection in patients' sera by mass spectrometry during two outbreaks of type A Botulism in France.
机构信息
Institut Pasteur, Unité des Bactéries Anaérobies et Toxines, Paris, France.
出版信息
J Clin Microbiol. 2012 Dec;50(12):4091-4. doi: 10.1128/JCM.02392-12. Epub 2012 Sep 19.
Abstract
In two outbreaks of food-borne botulism in France, Clostridium botulinum type A was isolated and characterized from incriminated foods. Botulinum neurotoxin type A was detected in the patients' sera by mouse bioassay and in vitro endopeptidase assay with an immunocapture step and identification of the cleavage products by mass spectrometry.
摘要
在法国的两起因食物传播的肉毒中毒暴发事件中，从可疑食物中分离并鉴定出 A 型肉毒梭菌。通过小鼠生物测定法和体外内切酶测定法（免疫捕获步骤），从患者血清中检测到 A 型肉毒神经毒素，并通过质谱法鉴定裂解产物。
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