Kusama Yusuke, Takayanagi Sayuri, Tategu Moe, Yoshida Kenichi
Department of Life Sciences, Faculty of Agriculture, Meiji University, Kanagawa 214-8571, Japan.
Exp Ther Med. 2010 Mar;1(2):395-399. doi: 10.3892/etm_00000062. Epub 2010 Mar 1.
The armadillo family of proteins has been implicated in embryogenesis and tumorigenesis. Armadillo repeat containing X-linked (ARMCX)1-6 and its most closely related protein, ARMC10, share an uncharacterized domain in their carboxyl-terminal region and thereby constitute a unique subfamily. We previously detected an elevated ARMCX6 mRNA level in human cervical carcinoma (HeLa) cells in which the cyclin-dependent kinase inhibitor 2A products (p16INK4A and p14ARF) were silenced by RNA interference; however, the function of the ARMCX6 gene has yet to be determined. In the present study, we demonstrated that ARMCX6 tagged with green fluorescent protein was localized in the cytoplasm of HeLa cells. ARMCX6 mRNA was highly detected in the pancreas and spleen, indicating a tissue-specific expression pattern in human tissues. Taken together, these results suggest that ARMCX6 may be uniquely involved in a specific cellular function of certain tissues.
犰狳蛋白家族与胚胎发育和肿瘤发生有关。含犰狳重复序列的X连锁蛋白（ARMCX）1 - 6及其关系最密切的蛋白ARMC10在其羧基末端区域共享一个未被表征的结构域，从而构成一个独特的亚家族。我们之前在人宫颈癌（HeLa）细胞中检测到ARMCX6 mRNA水平升高，在这些细胞中，细胞周期蛋白依赖性激酶抑制剂2A产物（p16INK4A和p14ARF）通过RNA干扰被沉默；然而，ARMCX6基因的功能尚未确定。在本研究中，我们证明用绿色荧光蛋白标记的ARMCX6定位于HeLa细胞的细胞质中。在胰腺和脾脏中高度检测到ARMCX6 mRNA，表明其在人体组织中具有组织特异性表达模式。综上所述，这些结果表明ARMCX6可能独特地参与某些组织的特定细胞功能。
