Grześk Elżbieta, Kołtan Sylwia, Dębski Robert, Wysocki Mariusz, Gruszka Marzena, Kubicka Małgorzata, Kołtan Andrzej, Grześk Grzegorz, Manysiak Sławomir, Odrowąż-Sypniewska Grażyna
Departments of Pediatrics, Hematology and Oncology.
Laboratory Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-094 Bydgoszcz, Poland.
Exp Ther Med. 2010 Sep;1(5):873-877. doi: 10.3892/etm.2010.119. Epub 2010 Jul 20.
The pathogenesis of viral bronchiolitis is poorly understood. The aim of this study was to analyze interleukin (IL)-15, IL-18 and interferon (IFN)-γ concentrations and the activity of NK cells and CD4 and CD8 lymphocytes in 23 children not older than 30 months of age with acute viral bronchiolitis using blood samples drawn within the first 24 h of their hospital admission, in comparison to a healthy group. In children with bronchiolitis, the mean concentrations of IL-15, IL-18 and IFN-γ were 9.39±11.55, 884.03±645.44 and 17.92±27.14 pg/ml, respectively, and were significantly higher than those in the control group [2.34±0.61 pg/ml (p<0.05), 248.69±98.73 pg/ml (p<0.001) and 2.75±1.72 pg/ml (p<0.005), respectively]. In the bronchiolitis group, mean z-scores were -1.15±1.9 for CD4 cells and -0.9±1.23 for CD8 cells; these scores were significantly lower than those of the general Polish population (p<0.001 and <0.01, respectively). However, the mean z-score of the ratio of CD4/CD8 and the NK cell count in children with bronchiolitis did not differ significantly from those of the controls. In conclusion, cytokines such as IL-15, IL-18 and IFN-γ play a role in the pathogenesis of bronchiolitis in children.
病毒性细支气管炎的发病机制尚不清楚。本研究的目的是分析23名年龄不超过30个月的急性病毒性细支气管炎患儿在入院后24小时内采集的血样中白细胞介素（IL）-15、IL-18和干扰素（IFN）-γ的浓度以及自然杀伤（NK）细胞、CD4和CD8淋巴细胞的活性，并与健康组进行比较。在细支气管炎患儿中，IL-15、IL-18和IFN-γ的平均浓度分别为9.39±11.55、884.03±645.44和17.92±27.14 pg/ml，显著高于对照组[分别为2.34±0.61 pg/ml（p<0.05）、248.69±98.73 pg/ml（p<0.001）和2.75±1.72 pg/ml（p<0.005）]。在细支气管炎组中，CD4细胞的平均z值为-1.15±1.9，CD8细胞的平均z值为-0.9±1.23；这些值显著低于波兰普通人群（分别为p<0.001和<0.01）。然而，细支气管炎患儿CD4/CD8比值的平均z值和NK细胞计数与对照组无显著差异。总之，IL-15、IL-18和IFN-γ等细胞因子在儿童细支气管炎的发病机制中起作用。
