University Children's Hospital Munich, Munich, Germany.
Allergy. 2012 Dec;67(12):1565-71. doi: 10.1111/all.12028. Epub 2012 Sep 21.
We have previously found an inverse association of bacterial diversity with childhood asthma. It remains unclear whether certain bacteria account for the protective effect.
The high variability of the bacterial 16S rRNA gene allows assessing diversity and specificity of bacterial communities by single-strand configuration polymorphism (SSCP). DNA was extracted from mattress dust samples of 489 school-age children from rural and suburban regions in Germany. A fragment of the bacteria-specific 16S rRNA gene was amplified by PCR, digested to single-strand DNA, and subjected to electrophoresis. The resulting band patterns reflect the underlying DNA sequences. The individual bands were tested for associations with asthma, hay fever, and atopy in quantitative and qualitative multivariable analyses. Significantly associated bands were isolated and sequenced. The sequences were compared to a database, and distinct bacteria were identified.
Seven of 76 independent bands were found to be inversely associated with asthma, atopic sensitization, and hay fever with odds ratios ranging from 0.17 to 0.73. The bands contained the sequences of Acinetobacter sp., Lactobacillus spp., Neisseria spp., Staphylococcus sciuri, Jeotgalicoccus sp., Corynebacterium spp., and others.
In a diverse microbial environment, certain bacteria may account for the protective effect on the development of asthma and atopy.
我们之前发现细菌多样性与儿童哮喘呈负相关。但仍不清楚是否有特定的细菌起到了保护作用。
细菌 16S rRNA 基因的高度变异性允许通过单链构象多态性（SSCP）评估细菌群落的多样性和特异性。从德国农村和郊区的 489 名学龄儿童的床垫灰尘样本中提取 DNA。通过 PCR 扩增细菌特异性 16S rRNA 基因的片段，将其消化为单链 DNA，并进行电泳。所得条带模式反映了潜在的 DNA 序列。对个体条带进行定量和定性多变量分析，以检测其与哮喘、花粉热和过敏的关联。对有显著关联的条带进行分离和测序。将这些序列与数据库进行比较，并鉴定出不同的细菌。
在 76 个独立条带中，有 7 个与哮喘、过敏致敏和花粉热呈负相关，比值比范围为 0.17 至 0.73。这些条带包含了不动杆菌属、乳杆菌属、奈瑟菌属、松鼠葡萄球菌、詹氏甲烷球菌、棒状杆菌属和其他细菌的序列。
在多样化的微生物环境中，某些细菌可能对哮喘和过敏的发展起到保护作用。
