Giri Shiv Kumar, Yadav Anita, Kumar Anil, Dev Kapil, Gulati Sachin, Gupta Ranjan, Aggarwal Neeraj, Gautam Sanjeev Kumar
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
Asian Pac J Cancer Prev. 2012;13(7):3409-16. doi: 10.7314/apjcp.2012.13.7.3409.
It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coal-tar workers exposed to PAHs in their work place.
Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects.
Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects.
In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt) +homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.
众所周知，多环芳烃（PAHs）如苯并（a）芘具有致癌特性，可能会导致人群患多种癌症。遗传易感性可能归因于编码致癌物代谢酶的基因变异，如细胞色素P - 450（CYP450）。我们的研究旨在调查细胞色素P450 1A1（CYP1A1，m1和m2）基因多态性对115名在工作场所接触多环芳烃的煤焦油工人遗传损伤的影响。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法测定CYP1A1基因多态性。采用彗星试验和口腔微核试验评估115名煤焦油工人和105名对照者的遗传损伤情况。
无论是CYP1A1 m1还是CYP1A1 m2的杂合子和纯合子（野生型/突变型+突变型/突变型）变异单独以及协同作用，与对照组和暴露组中通过尾矩（TM）和口腔微核（BMN）频率测量的遗传损伤均显示出显著相关性（P<0.05） 。
在我们的研究中，我们发现CYP1A1 m1和m2杂合子（野生型/突变型）+纯合子（突变型/突变型）变异与遗传损伤存在显著相关性，表明这些多态性可能会调节职业环境中多环芳烃暴露的影响。
