Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.
Bioresour Technol. 2012 Nov;124:355-63. doi: 10.1016/j.biortech.2012.08.055. Epub 2012 Aug 24.
This study determined the most effective microbes acting as ammonia-oxidising (AOB) and manganese-oxidising bacteria (MnOB) for the simultaneous removal of ammonia (NH(4)(+)-N) and manganese (Mn(2+)) from water. Two conditions of mixed culture of bacteria: an acclimatised mixed culture (mixed culture: MC) in a 5-L bioreactor and biofilm attached on a plastic medium (stages of mixed culture: SMC) in a biological aerated filter were isolated and identified using Biolog MicroSystem and 16S rRNA sequencing. A screening test for determining the most effective microbe in the removal of NH(4)(+)-N and Mn(2+) was initially performed using SMC and MC, respectively, and found that Bacillus cereus was the most effective microbe for the removal of NH(4)(+)-N and Mn(2+). Moreover, the simultaneous NH(4)(+)-N and Mn(2+) removal (above 95% removal for both NH(4)(+)-N and Mn(2+)) was achieved using a biological aerated filter under various operating conditions. Thus, the strain could act as an effective microbe of AOB and a MnOB for the simultaneous removal of NH(4)(+)-N and Mn(2+).
本研究旨在确定最有效的微生物，用于同时去除水中的氨（NH(4)(+)-N）和锰（Mn(2+)）。通过使用 Biolog MicroSystem 和 16S rRNA 测序，从 5-L 生物反应器中的驯化混合培养物（混合培养物：MC）和生物曝气滤池中的附着生物膜（混合培养物的阶段：SMC）中分离和鉴定了两种混合细菌培养条件。使用 SMC 和 MC 分别进行了筛选试验，以确定去除 NH(4)(+)-N 和 Mn(2+)的最有效微生物，结果发现蜡状芽孢杆菌是去除 NH(4)(+)-N 和 Mn(2+)的最有效微生物。此外，在各种操作条件下，生物曝气滤池可以实现 NH(4)(+)-N 和 Mn(2+)的同时去除（NH(4)(+)-N 和 Mn(2+)的去除率均超过 95%）。因此，该菌株可以作为 AOB 和 MnOB 的有效微生物，用于同时去除 NH(4)(+)-N 和 Mn(2+)。
