Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.
Bioresour Technol. 2012 Nov;124:387-93. doi: 10.1016/j.biortech.2012.08.048. Epub 2012 Aug 19.
Eukaryotic and bacterial communities were characterized and quantified in microalgal photobioreactor cultures of freshwater Chlorella vulgaris and marine Dunaliella tertiolecta. The microalgae exhibited good growth, whilst both cultures contained diverse bacterial communities. Both cultures included Proteobacteria and Bacteroidetes, while C. vulgaris cultures also contained Actinobacteria. The bacterial genera present in the cultures were different due to different growth medium salinities and possibly different extracellular products. Bacterial community profiles were relatively stable in D. tertiolecta cultures but not in C. vulgaris cultures likely due to presence of ciliates (Colpoda sp.) in the latter. The presence of ciliates did not, however, cause decrease in total number of C. vulgaris or bacteria during 14 days of cultivation. Quantitative PCR (qPCR) reliably showed relative microalgal and bacterial cell numbers in the batch cultures with stable microbial communities, but was not effective when bacterial communities varied. Raw culture samples were successfully used as qPCR templates.
在淡水小球藻和海洋杜氏盐藻的微藻光生物反应器培养物中，对真核生物和细菌群落进行了特征描述和定量分析。微藻表现出良好的生长，而两种培养物都含有多样化的细菌群落。两种培养物都包含变形菌门和拟杆菌门，而小球藻培养物还含有放线菌门。由于不同的生长培养基盐度和可能不同的胞外产物，培养物中存在的细菌属不同。由于后者中存在纤毛虫（胶须虫属），杜氏盐藻培养物中的细菌群落特征相对稳定，但小球藻培养物中的细菌群落特征则不稳定。然而，纤毛虫的存在并没有导致小球藻或细菌总数在 14 天的培养过程中减少。在具有稳定微生物群落的分批培养中，定量聚合酶链式反应（qPCR）可靠地显示了相对微藻和细菌细胞数量，但当细菌群落发生变化时，qPCR 则无效。原始培养物样品可成功用作 qPCR 模板。
