Comparison of the Performance and Microbial Community Structure of Two Outdoor Pilot-Scale Photobioreactors Treating Digestate.

This study aimed at examining and comparing the nutrient removal efficiency, biomass productivity and microbial community structure of two outdoor pilot-scale photobioreactors, namely a bubble column and a raceway pond, treating the liquid fraction of an agricultural digestate. Bacterial and eukaryotic communities were characterized using a metabarcoding approach and quantitative PCR. The abundance, composition, diversity, and dynamics of the main microbes were then correlated to the environmental conditions and operational parameters of the reactors. Both photobioreactors were dominated either by sp. or sp. in function of temperature, irradiance and the nitrogen compounds derived by nitrification. Other species, such as and , were sporadically present, demonstrating that they have more specific niche requirement. sp. always dominated the bacterial community in both reactors, except in summertime, when a bloom of occurred in the raceway pond. In autumn, the worsening of the climate conditions decreased the microalgal growth, promoting predation by sp. The study highlights the factors influencing the structure and dynamics of the microbial consortia and which ecological mechanisms are driving the microbial shifts and the consequent reactor performance. On these bases, control strategies could be defined to optimize the management of the microalgal-based technologies.