钙平衡作用：微管末端与晶格结合开关在 BPAG1n4 的羧基末端起作用。
Calcium tips the balance: a microtubule plus end to lattice binding switch operates in the carboxyl terminus of BPAG1n4.
机构信息
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, MSLS Building, Room P259, 1201 Welch Road, Stanford, California 94305, USA.
出版信息
EMBO Rep. 2012 Nov 6;13(11):1021-9. doi: 10.1038/embor.2012.140. Epub 2012 Sep 21.
Abstract
Microtubules (MTs) are integral to numerous cellular functions, such as cell adhesion, differentiation and intracellular transport. Their dynamics are largely controlled by diverse MT-interacting proteins, but the signalling mechanisms that regulate these interactions remain elusive. In this report, we identify a rapid, calcium-regulated switch between MT plus end interaction and lattice binding within the carboxyl terminus of BPAG1n4. This switch is EF-hand dependent, and mutations of the EF-hands abolish this dynamic behaviour. Our study thus uncovers a new, calcium-dependent regulatory mechanism for a spectraplakin, BPAG1n4, at the MT plus end.
摘要
微管（MTs）是许多细胞功能的组成部分，例如细胞黏附、分化和细胞内运输。它们的动力学主要由各种 MT 相互作用蛋白控制，但调节这些相互作用的信号机制仍然难以捉摸。在本报告中，我们确定了 BPAG1n4 羧基末端中 MT 正极相互作用和晶格结合之间的快速、钙调节开关。该开关依赖于 EF 手，EF 手的突变会消除这种动态行为。因此，我们的研究揭示了 MT 正极处 spectrin 结合蛋白 BPAG1n4 的一种新的、依赖钙的调节机制。
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