Stoffolano J G, Angioy A M, Crnjar R, Liscia A, Pietra P
Department of Entomology, University of Massachusetts, Amherst 01003.
J Med Entomol. 1990 Jan;27(1):14-23. doi: 10.1093/jmedent/27.1.14.
A modified behavioral test for determining the median tarsal acceptance thresholds of field-collected, female Tabanus nigrovittatus Macquart to sugars showed that flies were sensitive to the three major nectar sugars (fructose, sucrose, and glucose, in that order of decreasing sensitivity) whereas galactose, a nonnectar sugar, was not comparably stimulating. Comparisons of various treatment effects on the median tarsal acceptance thresholds showed that for future studies, flies should be fed 10% sucrose before testing, should be starved for 24 h before testing, and they do not have to be pretested for a water response. Electrophysiological recordings showed that gustatory chemosensilla were located on the labellum, tip of labrum, labral food canal, foretarsi, tibia, and antennae. All chemosensilla responded to sugar and salt, whereas only those chemosensilla at the tip of the labrum and those lining the food canal, which normally contact the blood meal, responded to sera and plasma.
一种经过改良的行为测试用于确定野外采集的雌性黑纹牛虻（Tabanus nigrovittatus Macquart）对糖类的跗节接受阈值中位数，结果表明，这些苍蝇对三种主要的花蜜糖类（果糖、蔗糖和葡萄糖，敏感性依次降低）敏感，而半乳糖这种非花蜜糖类则没有类似的刺激作用。对各种处理对跗节接受阈值中位数的影响进行比较后发现，对于未来的研究而言，在测试前应给苍蝇喂食10%的蔗糖，在测试前应使其饥饿24小时，并且无需对其进行水反应的预测试。电生理记录显示，味觉化学感受器位于唇瓣、上唇尖端、上唇食物通道、前跗节、胫节和触角上。所有化学感受器都对糖和盐有反应，而只有上唇尖端以及通常接触血餐的食物通道内衬的那些化学感受器对血清和血浆有反应。
