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通过原位杂交和三维光学显微镜对完整植物细胞核中的核糖体和端粒DNA序列进行定位。

Localization of ribosomal and telomeric DNA sequences in intact plant nuclei by in-situ hybridization and three-dimensional optical microscopy.

作者信息

Rawlins D J, Shaw P J

机构信息

Department of Cell Biology, John Innes Institute, Norwich, U.K.

出版信息

J Microsc. 1990 Jan;157(Pt 1):83-9. doi: 10.1111/j.1365-2818.1990.tb02949.x.

DOI:10.1111/j.1365-2818.1990.tb02949.x
PMID:2299663
Abstract

We have combined the use of three-dimensional (3-D) fluorescence microscopy and computer image processing of images with in-situ hybridization to analyse the 3-D organization of interphase nuclei in plants. In sections of root tips of Pisum sativum, using cDNA probes, we have shown that telomeres are arranged around the nuclear periphery and that the ribosomal genes in this species appear to exist in discrete, 3-D domains.

摘要

我们将三维（3-D）荧光显微镜技术与图像的计算机图像处理相结合，并运用原位杂交技术来分析植物间期细胞核的三维结构。在豌豆根尖切片中，使用cDNA探针，我们发现端粒围绕着核周排列，并且该物种中的核糖体基因似乎存在于离散的三维结构域中。

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Localization of ribosomal and telomeric DNA sequences in intact plant nuclei by in-situ hybridization and three-dimensional optical microscopy.通过原位杂交和三维光学显微镜对完整植物细胞核中的核糖体和端粒DNA序列进行定位。
J Microsc. 1990 Jan;157(Pt 1):83-9. doi: 10.1111/j.1365-2818.1990.tb02949.x.
2
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