Department of of Chemistry, Razi University, Kermanshah, Iran.
J Sep Sci. 2012 Oct;35(20):2718-24. doi: 10.1002/jssc.201200424. Epub 2012 Sep 20.
Dispersive liquid-liquid microextraction coupled with high-performance liquid chromatography-ultraviolet detection as a fast and inexpensive technique was applied to the simultaneous extraction and determination of traces of three common herbicides, 2,4-D, alachlor and atrazine, in aqueous samples. The critical experimental parameters, including type of the extraction and disperser solvents as well as their volumes, sample pH, salt addition, extraction time and centrifuging time, and speed were investigated and optimized. Under the optimum conditions, the calibration graphs found to be linear in the range of 0.3-200 μg/L with limits of detection in the range of 0.05-0.1 μg/L. The relative standard deviations were in the range of 4.5-6.2% (n = 7). The relative recoveries of well, tap, and river water samples which have been spiked with different levels of herbicides were 92.0-107.0, 82.0-104.0, and 82.0-86.0%, respectively.
分散液液微萃取结合高效液相色谱-紫外检测作为一种快速廉价的技术，被应用于水样中痕量的三种常见除草剂，2,4-D、甲草胺和莠去津的同时萃取和测定。考察并优化了关键的实验参数，包括萃取和分散溶剂的类型及其体积、样品 pH 值、盐的添加、萃取时间和离心时间及速度。在最佳条件下，校准曲线在线性范围内为 0.3-200μg/L，检出限在 0.05-0.1μg/L 范围内。相对标准偏差在 4.5-6.2%（n=7）范围内。对已加入不同水平除草剂的井水、自来水和河水样品进行加标回收实验，其回收率在 92.0-107.0%、82.0-104.0%和 82.0-86.0%之间。
